Nelson Lab Enzyme List (-20C Freezer)

Blue

Box #1

 

 

Blue

Box #2

 

 

Blue

Box #3

 

Index

Name

Company

 

Index

Name

Company

 

Index

Name

Company

1

Acc I

Promega

 

25

Dra I

Promega

 

49

Pfl MI

NEB

2

Acl I

NEB

 

26

Ecl I CRI

Promega

 

50

PshA I

NEB

3

Age I

NEB

 

27

Ecl136 II

Fermentas

 

51

Pst I

Promega

4

Alu I

Promega

 

28

Eco 47 III

 

 

52

Pvu I

Promega

5

AlwN I

NEB

 

29

Eco NI

NEB

 

53

Pvu II

Stratagene

6

Apa I

Promega

 

30

Eco O109

Stratagene

 

54

Rsa I

Promega

7

ApaL I

Stratagene

 

31

Eco RI

NEB

 

55

Sac I

Promega

8

Avr II

NEB

 

32

Eco RV

Promega

 

56

Sac II

Promega

9

BamHI

Promega

 

33

Hae II

NEB

 

57

Sal I

Promega

10

Bcl I

Promega

 

34

Hinc II

Stratagene

 

58

Sca I

Promega

11

Bgl I

Promega

 

35

Hind III

Promega

 

59

Sfi I

Promega

12

Bgl II

Promega

 

36

Hpa I

Stratagene

 

60

Sfu I

Roche

13

Bsa BI

NEB

 

37

Kpn I

Promega

 

61

Sma I

Promega

14

Bsm BI

NEB

 

38

Mbo I

Promega

 

62

Sna BI

Promega

15

Bsp 106

Stratagene

 

39

Mlu I

Promega

 

63

Spe I

Invitrogen

16

Bsp CI

Stratagene

 

40

Mwo I

NEB

 

64

Sph I

Promega

17

Bsp HI

NEB

 

41

Nae I

Promega

 

65

Ssp I

Promega

18

Bst EII

Promega

 

42

Nar I

Promega

 

66

Stu I

Promega

19

Bst XI

Promega

 

43

Nco I

Promega

 

67

Tth III

NEB

20

Bsu 36I

Promega

 

44

Nde I

 

 

68

Xba I

 

21

Cla I

NEB

 

45

Nhe I

NEB

 

69

Xho I

Invitrogen

22

Dpn I

Promega

 

46

Not I

Promega

 

70

Xma I

NEB

23

 

 

 

47

Nsi I

Promega

 

71

Xmn I

Promega

24

 

 

 

48

Nsp I

NEB

 

72

 

 

 

* Bold enzymes: an extra tube is in Green Box #4

 

 

Green

Box #4:

Backup Enzyme

 

Yellow

Box #5: Modifying Enzymes

 

Index

Name

Company

 

Index

Name

Company

73

Apa I

Promega

 

96

Alk. Phosphatase (CIP)

Promega

74

BamHI

NEB

 

97

Alk. Phosphatase (CIP)

Promega

75

Bgl I

Promega

 

98

Klenow

Takara

76

Bgl II

 

 

99

Pfu polymerase

Stratagene

77

BstEII

NEB

 

100

Pfu polymerase

Stratagene

78

Bsu 36I

Promega

 

101

Pfu polymerase

Stratagene

79

Bsu 36I

Promega

 

102

rRNasin (Rnase Inhibitor)

Promega

80

Dpn I

Promega

 

103

rRNasin (Rnase Inhibitor)

Promega

81

Eco 47III

Promega

 

104

RQ I Rnase-free Dnase

Promega

82

EcoRI

Promega

 

105

RQ I Rnase-free Dnase

Promega

83

EcoRV

NEB

 

106

rTdT

Gibco

84

Hinc II

Promega

 

107

TNT T7 Polymerase

Promega

85

Hind III

Promega

 

108

T4 DNA Polymerase

Promega

86

Kpn I

Promega

 

109

T4 Polynucl. Kinease

Promega

87

Mbo I

Promega

 

110

T4 Polynucl. Kinease

Takara

88

Not I

Promega

 

111

T4 Polynucl. Kinease

Takara

89

Pst I

Promega

 

112

T4 DNA Ligase HC

Promega

90

Sac I

NEB

 

113

T4 DNA Ligase Buffer

Invitrogen

91

Sac II

NEB

 

114

T4 DNA Ligase

Invitrogen

92

Sal I

Promega

 

115

dATP 10mM

 

93

Sca I

Promega

 

116

dATP 100mM

 

94

Sma I

 

 

117

dCTP 100mM

 

95

Spe I

Invitrogen

 

118

dGTP 100mM

 

96

Spe I

Promega

 

119

dTTP 100mM

 

 

 

 

 

 

 

 

White

Box:

Backup Enzyme

 

 

 

 

 

Sph I

Promega

 

 

 

 

 

Stu I

NEB

 

 

 

 

 

Xba I

NEB

 

 

 

 

 

Xho I

NEB

 

 

 

 

 

Xma I

NEB

 

 

 

 


Last Updated on 6/29/03
By Paige's little helper